Nucleic Acid Quantification
Calculate DNA or RNA concentration from A260 and the dilution factor, with optional A260/A280 purity.
🧪 Category
DNA & RNA
Nucleic acid quantification, melting temperature, and sequence utilities.
Nucleic acid work turns on a few recurring numbers: concentration from absorbance, mass from concentration and volume, melting temperature, and the conversions between them. This category groups calculators for A260-based quantitation, primer and oligo Tm, and DNA or RNA handling, with the unit conversions that sit alongside them. Results state their assumptions about purity and extinction coefficients rather than hiding them.
2 tools in this category
Frequently asked questions
How do I go from an A260 reading to setting up a PCR?
Convert the A260 absorbance to DNA or RNA concentration with the nucleic acid quantification calculator (it also reports the 260/280 purity ratio), check the primer melting temperature with the oligonucleotide Tm calculator, then scale per-reaction volumes for all tubes at once with the PCR master mix calculator.
What does the 260/280 ratio tell me before I continue?
It is a purity indicator for nucleic acid preparations: the quantification calculator reports it next to the concentration so you can judge whether the sample is clean enough for downstream work before spending reagents.